Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 855-1

855-1

COST-EFFECTIVE PRODUCTION OF FUNGAL BIOSURFACTANT USING WASTE-BASED FEEDSTOCKS AS ALTERNATIVE SUBSTRATES

Autores:

Dayana Montero Rodríguez (UNICAP - Universidade Católica de Pernambuco) ; Everton Ricardo Carneiro Costa (UNICAP - Universidade Católica de Pernambuco) ; Rafael de Souza Mendonça (UFRPE - Universidade Federal Rural de Pernambuco, UNICAP - Universidade Católica de Pernambuco) ; Allem Karolyne Dino da Silva (UNICAP - Universidade Católica de Pernambuco) ; Adriana Ferreira de Souza (UNICAP - Universidade Católica de Pernambuco) ; Ranilson de Souza Bezerra (UFPE - Universidade Federal de Pernambuco) ; Rosileide Fontenele da Silva Andrade (UNICAP - Universidade Católica de Pernambuco) ; Galba Maria de Campos Takaki (UNICAP - Universidade Católica de Pernambuco)

Resumo:

Microbial biosurfactants are surface-active molecules of increasing interest worldwide due to their low toxicity, high biodegradability and minimal ecological impact, in comparation with the synthetic ones. Although most researchers report that bacteria and yeast are the main microorganisms producing surfactant compounds, filamentous fungi also have excellent potential. In this sense, several studies have highlighted the multifunctional properties of fungal biosurfactants; however, their production and marketing are still affected by high production costs. Hence, this study focused on cost-effective production of biosurfactant by Mucorales fungus Rhizopus arrhizus UCP 1295, using two waste-based feedstocks as alternative substrates. For this, inexpensive media were formulated using salt solution supplemented with different concentrations of fishery-processing waste (FPW) and waste soybean oil (WSO), according to a 2² full-factorial design (FFD). Spores of R. arrhizus were used as inoculum for fermentative media, which were incubated at 28°C and 150 rpm, for 96 h. Then, the cultures were subjected to filtration and centrifugation and the cell-free metabolic liquids were used to determinate surface and interfacial tension by the Du Noüy ring method. Statistical analysis was performed in order to evaluate the influence of FPW and WSO concentration on surface and interfacial tension. According to the results, excellent biosurfactant production was exhibited in condition 4 of the FFD, with reduction of surface and interfacial tension of the medium to 26.6 and 0.8 mN/m, respectively. In addition, statistical analysis demonstrated the significant influence of both substrates on biosurfactant production. In conclusion, this study evidenced that the use of waste-based raw materials as low-cost substrates is a promising strategy to enhance the economic production and commercialization of fungal biosurfactants.

Palavras-chave:

Microbial surfactant, Mucorales fungus, Fishery-processing waste, Waste soybean oil

Agência de fomento:

CAPES (PNPD fellowship, Process no. 88882.314590/2019-01), FACEPE and CNPq